Abstract : Background: Thyroid peroxidase (TPO) enzyme is required for the synthesis of thyroid hormones. Any
alteration in TPO results in a deranged thyroid profile. Antibodies against TPO serve as useful markers for
the detection of auto-immune thyroid diseases. Thus, present study was conducted to analyse the relation
of anti-TPO antibodies with thyroid dysfunction.
Materials and Methods: After obtaining an ethical approval, this cross-sectional study was carried out for
a period of 2 years. Adult patients (18 years old) presenting with symptoms related to thyroid dysfunction
and referred for analysis of T3, T4, TSH and anti-TPO antibodies were included in the study. Over night
fasting blood samples were collected, labelled and stored. Anti-TPO antibody test for human IgG was
done using Enzyme Linked Immunosorbent Assay (ELISA) kit. Statistical analysis was carried out using
SPSS (version 26.0). Chi square test was used to compare the data and p-value
Results: Total 100 patients were analyzed. Maximum 32 patients were found in 21-30 year age group.
Mean age for females was 33.7 1.20 years. Male: female ratio was 1: 2.12. Hypothyroid was noted in
maximum 73 patients with 49 females and 24 males. Positive anti-TPO antibody patients were 44 out of
which 33 were females. Almost 35 out of 44 patients were anti-TPO antibody positive and hypothyroid with
majority 27 females. Maximum12 out of 27 hypothyroid and anti-TPO antibody positive females belonged
to 21-30 year age group.
Conclusion: Anti-TPO antibodies were found to be associated with hypothyroid dysfunction. It was noted
more among females of reproductive age group.
Keywords: Anti-TPO antibodies, Females, Hypothyroid, Thyroid peroxidase enzyme.