Indian Journal of Microbiology Research

Innovative Education and Scientific Research Foundation

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Registered Under Government of India NGO – Indian Trust Act 1882

Bacteriological profile of pus samples and their antibiotic susceptibility pattern

Article : Open access

Article type : Research Article

Volume : 7

Issue : 1

Year : 2020

Author : Mita D Wadekar, Sathish J V, Jayashree, Pooja C

Page no : 43-47

Abstract : Introduction: Pyogenic infections can be caused by various microorganisms and mixed infections are common which require antibiotic therapy. The inappropriate use of antibiotics has resulted in development of antibiotic resistance. The bacteriological profile may remain same, but antibiotic susceptibility pattern varies. Hence the study was conducted to know bacteriological profile of pus samples and their antibiotic susceptibility pattern. Materials and Methods: A retrospective study was carried out from January to June 2019. 108 pus samples collected during study period were included. The samples were cultured on Blood and MacConkey agar. After aerobic incubation at 37oC for 18-24 hrs, organisms were identified by standard methods and antibiotic susceptibility was tested by Kirby Bauer disc diffusion method. ESBL was detected by combined disk test and Methicillin resistant Staphylococcus aureus (MRSA) by Cefoxitin 30 mg disc. Results: Of 118 pus samples collected, 101(85.5%) were positive cultures and no growth was in 17(14.4%) samples. S. aureus 27(22.9%) was most common Gram positive isolate and Pseudomonas spp. 17(14.4%), was most common gram negative isolate. ESBL positivity was seen in 38(61.2%) and MRSA in 13(48.1%) S. aureus isolates. Most of gram negative isolates were susceptible to piperacillin / tazobactum 55(88.7%) and meropenem 53(85.4%) and gram positive isolates to vancomycin 27(100%) and linezolid 25(92.5%). Conclusion: The spread of beta-lactamase producing organisms has been increasing. Our study showed increased resistance to beta-lactam antibiotics which is a serious problem. To combat resistance irrational use of antibiotics should be avoided. Also regular surveillance helps in implementing better therapeutic strategies to reduce morbidity and mortality. Keywords: Pus, Bacteriological profile, ESBL, MRSA.