Indian Journal of Microbiology Research

Innovative Education and Scientific Research Foundation

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Registered Under Government of India NGO – Indian Trust Act 1882

Mechanisms of carbapenem resistance among clinical isolates of escherichia coli in a tertiary care hospital

Article : Open access

Article type : Research Article

Volume : 7

Issue : 1

Year : 2020

Author : Nikhita C Ved, Rudresh S M, Ravi G S, Malini J, Raksha Yoganand

Page no : 70-75

Abstract : Introduction: The emergence and global spread of carbapenem-resistant E. coli is of great concern to the health care facilities due to high rates of morbidity and mortality associated with them. It is necessary to know its epidemiology and the resistant pattern in a geographical area to formulate a antibiotic stewardship policy. Objective: To study the occurrence and mechanisms of carbapenem resistance in Escherichia coli. Materials and Methods: A total of 1172 clinical strains of E. coli obtained from various clinical specimens were screened for carbapenem resistance during the study period. Strains showing reduced susceptibility to imipenem &/or ertapenem &/or meropenem were included in the study. The resistance mechanisms were identified using various phenotypic methods. Results: Total of 53/1172 were found to be carbapenem resistant E. coli (CRE). The most common sample in which CRE were isolated was urine (n=26, 49.1%). A total of 50 isolates were confirmed as Metallobeta- lactamase (MBL) producers using Ezy MICTM strip. One of the three non carbapenemase producing isolate was positive for ESBL with porin loss and the other two isolates were positive for AmpC with porin loss. Conclusion: MBL production being the most common mechanism of carbapenem resistance, the study indicates the importance of regular monitoring of drug resistance in the hospital for an urgent action to be taken for antibiotics stewardship in the institute. Keywords: Escherichia coli, Carbapenem resistance, Super bug, Carba NP, MBL, Carbapenem resistance mechanisms.